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Mendeley Ltd raw western blot and microscopy images
Raw Western Blot And Microscopy Images, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Accessibility of the Shine-Dalgarno Sequence Dictates N-Terminal Codon Bias in E. coli
Article Snippet: .. Western blot raw images , Mendeley link , https://data.mendeley.com/datasets/9p7798pkks/1. ..

Article Title: Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pat-3 RNAi Gift from A. Wehman N/A Pdyf-11:UNC-23B This Study pAS169 Pdpy-30:UNC-23B This Study pAS157 Pdpy-30:UNC-23C This Study pAS161 Pdyf-11:UNC-23B This study pAS169 Pmir-228:UNC-23B:SL2:mCherry This Study pAS172 Prab-3:UNC-23B This Study pAS173 Punc-23A:GFP This Study pAS180 Pdpy-30:UNC-23B(E238R This Study pAS186 Pdpy-30:unc23B(E238K) This study pAS188 Pdpy-30:hBAG3 This Study pAS313 Pdpy-30:hBAG1 This Study pAS408/JF9 Psrtx-1:HisCation:SL2:GFP This study pAS537 Pdpy-30:hBAG2 This Study ASJ310/pCF11 Prol-6:UNC-23B:SL2:mCherry This study ASJ101/pCM16 Pcol-19:UNC-23B:SL2:mCherry This study ASJ64/pCM3 Pdpy-7:PH-PLCdelta:GFP This study ASJ509/pJB13 Pmyo-3:UNC-23B:SL2:GFP This study ASJ491/pJB7 Psrtx-1B:UNC-23B:SL2:mCherry This Study pJF27/pAS426 Pdpy-30:UNC-23A This Study pJF39 PF53F4.13:UNC-23B:SL2:mCherry This Study pJF71 Pdpy-30:hBAG1 This Study pJF9 PF53F4.13:PH-PLCdelta:GFP This Study pOO1 PF53F14.13:KCC-3:mSc Ray et al. 63 pSR7 unc-23 fosmid Transgeneome WRM0612aC08 sma-1 fosmid Transgeneome WRM066D12 Software and algorithms Prism 9.4 Graphpad https://www.graphpad.com/ FIJI ImageJ https://fiji.sc/ ApE Wayne Davis https://jorgensen.biology. utah.edu/wayned/ape/ Other PluriSelect Cell Strainer (L4) N/A TGX stain-free Precast Gels Bio-Rad Cat#4568094 Transblot Turbo Transfer system Bio-Rad Cat#1704150 Trans-blot Turbo transfer membrane Bio-Rad Cat#1704158 ChemiDoc MP imaging system Bio-Rad Cat#12003154 H-bridge amplifier Accuthermo Cat#AFTX700D Temperature controller Accuthermo Cat#FTC200 Thermistor McShane Cat#TR91-170 e3 Developmental Cell 59, 1–21.e1–e7, July 8, 2024 .. d All data reported in this paper will be shared by the lead contact upon request. d This paper does not report original code. d Any additional information required to reanalyze the data reported in this work paper is available from the lead contact upon request. d Western blot raw images: Mendeley Data https://doi.org/10.17632/6k4bfygcs2.1 ..

Article Title: Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans.
Article Snippet: .. Western blot raw images available at Mendeley Data, https://doi.org/10.17632/6k4bfygcs2.1. ..

Article Title: Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans
Article Snippet: .. Western blot raw images: Mendeley Data DOI: 10.17632/6k4bfygcs2.1 ..

Article Title: Co-transcriptional genome surveillance by HUSH is coupled to termination machinery
Article Snippet: ChIP-seq, RNA-seq, and PRO-seq , This paper , GEO: GSE208753. .. Western blot raw images , This paper , Mendeley DOI: 0.17632/v5y87syfcc.1. ..

Article Title: Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans
Article Snippet: .. Western blot raw images available at Mendeley Data, DOI: 10.17632/6k4bfygcs2.1 Worms were immobilized with 40mM sodium azide. .. Images were collected on a Deltavision Elite RoHS wide-field deconvolution system with Ultimate Focus (GE), a PlanApo 60×/1.42 NA or OLY 100×/1.40 NA oil-immersion objective and a DV Elite CMOS Camera.

Article Title: Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans
Article Snippet: .. Western blot raw images: Mendeley Data DOI: 10.17632/6k4bfygcs2.1 C. elegans hermaphrodites were used in all reported experiments and were cultured as previously described 69 , 166 . ..

Article Title: Mutations in the transcription factor FOXO1 mimic positive selection signals to promote germinal center B cell expansion and lymphomagenesis.
Article Snippet: .. All Western Blot raw images have been deposited in Mendeley Data: https://data.mendeley.com/datasets/ xfv7v9k45r/1. e6 Immunity 54, 1807–1824.e1–e14, August 10, 2021 ..

Cell Culture:

Article Title: Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans
Article Snippet: .. Western blot raw images: Mendeley Data DOI: 10.17632/6k4bfygcs2.1 C. elegans hermaphrodites were used in all reported experiments and were cultured as previously described 69 , 166 . ..



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Raw Western Blot And Microscopy Images, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MMDD suppresses tumor growth via SIRT1 downregulation and enhanced p65 acetylation (A and C) <t>Western</t> <t>blot</t> analysis of SIRT1 and acetyl-p65 protein levels in tumor tissues from vehicle control (sterile deionized water), L-MMDD, M-MMDD, and H-MMDD groups. GAPDH served as the internal loading control for SIRT1, acetyl-p65, and p65. (B and D) Quantitative analysis of relative SIRT1 and acetyl-p65/p65 expression levels in tumor tissues using ImageJ software ( n = 3). (E) Representative immunofluorescence <t>images</t> of SIRT1 (red) and acetyl-p65 (green) in tumor sections. Nuclei were counterstained with DAPI (blue) (scale bar, 50 μm). (F) Quantification of mean fluorescence intensity for SIRT1 and acetyl-p65 ( n = 3). (G) ELISA quantification of cytokine levels (IL-1β, IL-6, IL-12, TNF-α, and IFN-γ) in tumor homogenates ( n = 6). Data are represented as mean ± SD. One-way ANOVA was used for comparison among multiple groups.∗ p < 0.05 and ∗∗ p < 0.01.
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MMDD suppresses tumor growth via SIRT1 downregulation and enhanced p65 acetylation (A and C) <t>Western</t> <t>blot</t> analysis of SIRT1 and acetyl-p65 protein levels in tumor tissues from vehicle control (sterile deionized water), L-MMDD, M-MMDD, and H-MMDD groups. GAPDH served as the internal loading control for SIRT1, acetyl-p65, and p65. (B and D) Quantitative analysis of relative SIRT1 and acetyl-p65/p65 expression levels in tumor tissues using ImageJ software ( n = 3). (E) Representative immunofluorescence <t>images</t> of SIRT1 (red) and acetyl-p65 (green) in tumor sections. Nuclei were counterstained with DAPI (blue) (scale bar, 50 μm). (F) Quantification of mean fluorescence intensity for SIRT1 and acetyl-p65 ( n = 3). (G) ELISA quantification of cytokine levels (IL-1β, IL-6, IL-12, TNF-α, and IFN-γ) in tumor homogenates ( n = 6). Data are represented as mean ± SD. One-way ANOVA was used for comparison among multiple groups.∗ p < 0.05 and ∗∗ p < 0.01.
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MMDD suppresses tumor growth via SIRT1 downregulation and enhanced p65 acetylation (A and C) <t>Western</t> <t>blot</t> analysis of SIRT1 and acetyl-p65 protein levels in tumor tissues from vehicle control (sterile deionized water), L-MMDD, M-MMDD, and H-MMDD groups. GAPDH served as the internal loading control for SIRT1, acetyl-p65, and p65. (B and D) Quantitative analysis of relative SIRT1 and acetyl-p65/p65 expression levels in tumor tissues using ImageJ software ( n = 3). (E) Representative immunofluorescence <t>images</t> of SIRT1 (red) and acetyl-p65 (green) in tumor sections. Nuclei were counterstained with DAPI (blue) (scale bar, 50 μm). (F) Quantification of mean fluorescence intensity for SIRT1 and acetyl-p65 ( n = 3). (G) ELISA quantification of cytokine levels (IL-1β, IL-6, IL-12, TNF-α, and IFN-γ) in tumor homogenates ( n = 6). Data are represented as mean ± SD. One-way ANOVA was used for comparison among multiple groups.∗ p < 0.05 and ∗∗ p < 0.01.
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MMDD suppresses tumor growth via SIRT1 downregulation and enhanced p65 acetylation (A and C) <t>Western</t> <t>blot</t> analysis of SIRT1 and acetyl-p65 protein levels in tumor tissues from vehicle control (sterile deionized water), L-MMDD, M-MMDD, and H-MMDD groups. GAPDH served as the internal loading control for SIRT1, acetyl-p65, and p65. (B and D) Quantitative analysis of relative SIRT1 and acetyl-p65/p65 expression levels in tumor tissues using ImageJ software ( n = 3). (E) Representative immunofluorescence <t>images</t> of SIRT1 (red) and acetyl-p65 (green) in tumor sections. Nuclei were counterstained with DAPI (blue) (scale bar, 50 μm). (F) Quantification of mean fluorescence intensity for SIRT1 and acetyl-p65 ( n = 3). (G) ELISA quantification of cytokine levels (IL-1β, IL-6, IL-12, TNF-α, and IFN-γ) in tumor homogenates ( n = 6). Data are represented as mean ± SD. One-way ANOVA was used for comparison among multiple groups.∗ p < 0.05 and ∗∗ p < 0.01.
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MMDD suppresses tumor growth via SIRT1 downregulation and enhanced p65 acetylation (A and C) Western blot analysis of SIRT1 and acetyl-p65 protein levels in tumor tissues from vehicle control (sterile deionized water), L-MMDD, M-MMDD, and H-MMDD groups. GAPDH served as the internal loading control for SIRT1, acetyl-p65, and p65. (B and D) Quantitative analysis of relative SIRT1 and acetyl-p65/p65 expression levels in tumor tissues using ImageJ software ( n = 3). (E) Representative immunofluorescence images of SIRT1 (red) and acetyl-p65 (green) in tumor sections. Nuclei were counterstained with DAPI (blue) (scale bar, 50 μm). (F) Quantification of mean fluorescence intensity for SIRT1 and acetyl-p65 ( n = 3). (G) ELISA quantification of cytokine levels (IL-1β, IL-6, IL-12, TNF-α, and IFN-γ) in tumor homogenates ( n = 6). Data are represented as mean ± SD. One-way ANOVA was used for comparison among multiple groups.∗ p < 0.05 and ∗∗ p < 0.01.

Journal: iScience

Article Title: Maimendong decoction suppresses non-small cell lung cancer growth by promoting dendritic cell maturation via the SIRT1/p65 acetylation pathway

doi: 10.1016/j.isci.2026.114774

Figure Lengend Snippet: MMDD suppresses tumor growth via SIRT1 downregulation and enhanced p65 acetylation (A and C) Western blot analysis of SIRT1 and acetyl-p65 protein levels in tumor tissues from vehicle control (sterile deionized water), L-MMDD, M-MMDD, and H-MMDD groups. GAPDH served as the internal loading control for SIRT1, acetyl-p65, and p65. (B and D) Quantitative analysis of relative SIRT1 and acetyl-p65/p65 expression levels in tumor tissues using ImageJ software ( n = 3). (E) Representative immunofluorescence images of SIRT1 (red) and acetyl-p65 (green) in tumor sections. Nuclei were counterstained with DAPI (blue) (scale bar, 50 μm). (F) Quantification of mean fluorescence intensity for SIRT1 and acetyl-p65 ( n = 3). (G) ELISA quantification of cytokine levels (IL-1β, IL-6, IL-12, TNF-α, and IFN-γ) in tumor homogenates ( n = 6). Data are represented as mean ± SD. One-way ANOVA was used for comparison among multiple groups.∗ p < 0.05 and ∗∗ p < 0.01.

Article Snippet: Raw western blot images , Mendeley Data , https://doi.org/10.17632/p9fkgm2wkj.2.

Techniques: Western Blot, Control, Sterility, Expressing, Software, Immunofluorescence, Fluorescence, Enzyme-linked Immunosorbent Assay, Comparison